nasD [2019-06-18 11:57:09]

assimilatory nitrite reductase (subunit)

Locus: BSU03300

Isoelectric point: 4.95

Molecular weight: 88.24 kDa

Protein length: 805 aa

Gene length: 2418 bp

Function: utilization of nitrite as nitrogen source

Product: assimilatory nitrite reductase (subunit)

Essential: no

E.C.: 1.7.1.4

Synonyms: nasBC,nirB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Respiration → Anaerobic respiration
• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of nitrate/ nitrite

Gene

Coordinates: 355,764 → 358,181

The protein

Catalyzed reaction/ biological activity

• Ammonium hydroxide + 3 NAD(P)⁺ + H₂O = nitrite + 3 NAD(P)H (according to Swiss-Prot)

Protein family

• nitrite and sulfite reductase 4Fe-4S domain family (according to Swiss-Prot)

Paralogous protein(s)

• NasB

Cofactors

• Fe-S cluster PubMed
• FAD PubMed

Expression and Regulation

Operons

• Genes: nasB-nasC-nasD-nasE-nasF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • TnrA: activation, PubMed, in TnrA regulon
  • NsrR: repression, PubMed, in NsrR regulon
  • Fur: repression, PubMed, in Fur regulon

  Regulation

  • nasD: expressed under anaerobic conditions (ResD) PubMed

• Genes: nasD-nasE-nasF
  Description: PubMed

  Regulation

  • nasD: expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• 1A973 ( nasD::phleo), PubMed, available at BGSC
• BKE03300 (ΔnasD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAGATGATCCGCTCCTT, downstream forward: _UP4_TAATGACAAAAACTATCATT
• BKK03300 (ΔnasD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAGATGATCCGCTCCTT, downstream forward: _UP4_TAATGACAAAAACTATCATT

References

Reviews

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Original publications

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